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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in several tumors, which leads to chromosome instability, and therefore might contribute to tumorigenesis. This protein was uncovered to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a critical job in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have already been described. Two added splice variants have been noted but detailed nucleotide sequence information is just not nevertheless offered.
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Blocking peptides are peptides that bind exclusively for the target antibody and block antibody binding. These peptide commonly consists of the epitope identified by the antibody.
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This guidebook outlines even more feasible optimization of mobile and immunoassay parameters to be certain the best possible benefits are...
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This guideline outlines further more possible optimization of cellular and immunoassay parameters to be certain the very best results are...
Antibodies bound to the blocking peptide now not bind into the epitope over the concentrate on protein. By evaluating the staining in the blocked antibody compared to the antibody by yourself, one can see which staining is certain.
Goal Info Cyclin E belongs to the highly conserved cyclin spouse and children, whose users are characterised by a remarkable periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic party. Cyclin E types a complex with and functions as being a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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